Optimization of salt concentration in PEG-based crystallization solutions

Although polyethylene glycol (PEG) is the most widely used precipitant in protein crystallization, the concentration of co-existing salt in the solution has not been well discussed. To determine the optimum salt concentration range, several kinds of protein were crystallized in a 30% PEG 4000 soluti...

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Autores principales: Yamanaka, Mari, Inaka, Koji, Furubayashi, Naoki, Matsushima, Masaaki, Takahashi, Sachiko, Tanaka, Hiroaki, Sano, Satoshi, Sato, Masaru, Kobayashi, Tomoyuki, Tanaka, Tetsuo
Formato: Artigo
Lenguaje:Inglês
Publicado: International Union of Crystallography 2010
Materias:
Diffraction Structural Biology
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC3004262/
https://ncbi.nlm.nih.gov/pubmed/21169699
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S0909049510035995
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