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Target RNA-directed tailing and trimming purifies the sorting of endo-siRNAs between the two Drosophila Argonaute proteins

In flies, 22–23-nucleotide (nt) microRNA duplexes typically contain mismatches and begin with uridine, so they bind Argonaute1 (Ago1), whereas 21-nt siRNA duplexes are perfectly paired and begin with cytidine, promoting their loading into Ago2. A subset of Drosophila endogenous siRNAs—the hairpin-de...

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Bibliografske podrobnosti
Main Authors: Ameres, Stefan L., Hung, Jui-Hung, Xu, Jia, Weng, Zhiping, Zamore, Phillip D.
Format: Artigo
Jezik:Inglês
Izdano: Cold Spring Harbor Laboratory Press 2011
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3004066/
https://ncbi.nlm.nih.gov/pubmed/21106652
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2498411
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