Identification and Functions of Amino Acid Residues in PotB and PotC Involved in Spermidine Uptake Activity

Amino acid residues on PotB and PotC involved in spermidine uptake were identified by random and site-directed mutagenesis. It was found that Trp(8), Tyr(43), Trp(100), Leu(110), and Tyr(261) in PotB and Trp(46), Asp(108), Glu(169), Ser(196), Asp(198), and Asp(199) in PotC were strongly involved in...

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Bibliografiska uppgifter
Huvudupphovsmän: Higashi, Kyohei, Sakamaki, Yoshiharu, Herai, Emiko, Demizu, Risa, Uemura, Takeshi, Saroj, Sunil D., Zenda, Risa, Terui, Yusuke, Nishimura, Kazuhiro, Toida, Toshihiko, Kashiwagi, Keiko, Igarashi, Kazuei
Materialtyp: Artigo
Språk:Inglês
Publicerad: American Society for Biochemistry and Molecular Biology 2010
Ämnen:
Membrane Biology
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC2998158/
https://ncbi.nlm.nih.gov/pubmed/20937813
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M110.186536
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