Identification and Functions of Amino Acid Residues in PotB and PotC Involved in Spermidine Uptake Activity

Amino acid residues on PotB and PotC involved in spermidine uptake were identified by random and site-directed mutagenesis. It was found that Trp(8), Tyr(43), Trp(100), Leu(110), and Tyr(261) in PotB and Trp(46), Asp(108), Glu(169), Ser(196), Asp(198), and Asp(199) in PotC were strongly involved in...

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Detalhes bibliográficos
Main Authors: Higashi, Kyohei, Sakamaki, Yoshiharu, Herai, Emiko, Demizu, Risa, Uemura, Takeshi, Saroj, Sunil D., Zenda, Risa, Terui, Yusuke, Nishimura, Kazuhiro, Toida, Toshihiko, Kashiwagi, Keiko, Igarashi, Kazuei
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2010
Assuntos:
Membrane Biology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2998158/
https://ncbi.nlm.nih.gov/pubmed/20937813
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M110.186536
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