Isolation and sequencing of the gene encoding delta 5-3-ketosteroid isomerase of Pseudomonas testosteroni: overexpression of the protein.

We describe the cloning, sequencing, and overexpression of the steroid isomerase (3-oxosteroid delta 5-delta 4-isomerase, EC 5.3.3.1) gene of Pseudomonas testosteroni. A genomic library of P. testosteroni total DNA constructed from partial EcoRI digests ligated to a lambda gtWES vector was probed wi...

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Autores principales: Kuliopulos, A, Shortle, D, Talalay, P
Formato: Artigo
Lenguaje:Inglês
Publicado: 1987
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC299657/
https://ncbi.nlm.nih.gov/pubmed/3480517
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