Identification of active site residues by site-directed mutagenesis of delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B.

In order to assess the roles of specific amino acid residues in the delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B during catalysis, we replaced aspartic acid 40 with asparagine (D40N) and tyrosine 16 with phenylalanine (Y16F) in the enzyme by site-directed mutagenesis. Both purif...

ver descrição completa

Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Kim, S W, Choi, K Y
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1995
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC176927/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7730300/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.177.9.2602-2605.1995
Tags:	Adicionar Tag Sem tags, seja o primeiro a adicionar uma tag!