Identification of active site residues by site-directed mutagenesis of delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B.
In order to assess the roles of specific amino acid residues in the delta 5-3-ketosteroid isomerase from Pseudomonas putida biotype B during catalysis, we replaced aspartic acid 40 with asparagine (D40N) and tyrosine 16 with phenylalanine (Y16F) in the enzyme by site-directed mutagenesis. Both purif...
Guardat en:
| Publicat a: | J Bacteriol |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1995
|
| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC176927/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7730300/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.177.9.2602-2605.1995 |
| Etiquetes: |
Sense etiquetes, Sigues el primer a etiquetar aquest registre!
|