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Four-color single molecule fluorescence with noncovalent dye labeling to monitor dynamic multimolecular complexes

To allow studies of conformational changes within multi-molecular complexes, we present a simultaneous, 4-color single molecule fluorescence methodology implemented with total internal reflection illumination and camera based, wide-field detection. We further demonstrate labeling histidine-tagged pr...

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Autors principals: DeRocco, Vanessa C., Anderson, Trevor, Piehler, Jacob, Erie, Dorothy A., Weninger, Keith
Format: Artigo
Idioma:Inglês
Publicat: 2010
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2996100/
https://ncbi.nlm.nih.gov/pubmed/21091445
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000113551
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