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Four-color single molecule fluorescence with noncovalent dye labeling to monitor dynamic multimolecular complexes
To allow studies of conformational changes within multi-molecular complexes, we present a simultaneous, 4-color single molecule fluorescence methodology implemented with total internal reflection illumination and camera based, wide-field detection. We further demonstrate labeling histidine-tagged pr...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2996100/ https://ncbi.nlm.nih.gov/pubmed/21091445 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2144/000113551 |
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