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Optimization of enzymatic reaction conditions for generating representative pools of cDNA from small RNA

Small regulatory RNA repertoires in biological samples are heterogeneous mixtures that may include species arising from varied biosynthetic pathways and modification events. Small RNA profiling and discovery approaches ought to capture molecules in a way that is representative of expression level. I...

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Detalhes bibliográficos
Main Authors: Munafó, Daniela B., Robb, G. Brett
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2010
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2995414/
https://ncbi.nlm.nih.gov/pubmed/20921270
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.2242610
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