Specific binding of transposase to terminal inverted repeats of transposable element Tn3.

Tn3 transposase, which is required for transposition of Tn3, has been purified by a low-ionic-strength-precipitation method. Using a nitrocellulose filter binding assay, we have shown that transposase binds to any restriction fragment. However, binding of the transposase to specific fragments contai...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Ichikawa, H, Ikeda, K, Wishart, W L, Ohtsubo, E
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1987
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC299513/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2825182/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.23.8220
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