Specific binding of transposase to terminal inverted repeats of transposable element Tn3.
Tn3 transposase, which is required for transposition of Tn3, has been purified by a low-ionic-strength-precipitation method. Using a nitrocellulose filter binding assay, we have shown that transposase binds to any restriction fragment. However, binding of the transposase to specific fragments contai...
Shranjeno v:
| izdano v: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
1987
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC299513/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2825182/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.23.8220 |
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