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Specific binding of transposase to terminal inverted repeats of transposable element Tn3.

Tn3 transposase, which is required for transposition of Tn3, has been purified by a low-ionic-strength-precipitation method. Using a nitrocellulose filter binding assay, we have shown that transposase binds to any restriction fragment. However, binding of the transposase to specific fragments contai...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Ichikawa, H, Ikeda, K, Wishart, W L, Ohtsubo, E
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1987
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC299513/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2825182/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.23.8220
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