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Tn5tac1, a derivative of transposon Tn5 that generates conditional mutations.

Conditional lethal mutations are valuable for analyzing essential genes. We describe here a derivative of the bacterial transposon Tn5 called Tn5tac1 and its use in an innovative strategy for making mutations with conditional phenotypes. The 4.6-kilobase Tn5tac1 element contains a strong, regulatabl...

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Détails bibliographiques
Publié dans:Proc Natl Acad Sci U S A
Auteurs principaux: Chow, W Y, Berg, D E
Format: Artigo
Langue:Inglês
Publié: National Academy of Sciences 1988
Sujets:
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC281994/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2842773/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.17.6468
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