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Use of the promoter fusion transposon Tn5 lac to identify mutations in Bordetella pertussis vir-regulated genes.

Mutants of Bordetella pertussis deficient in virulence-associated factors were identified by using the transposon Tn5 lac. Tn5 lac is a derivative of Tn5 which generates promoter fusions for beta-galactosidase. Tn5 lac insertions in the vir-regulated genes of B. pertussis were identified by selectin...

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Bibliografische gegevens
Hoofdauteurs: Weiss, A A, Melton, A R, Walker, K E, Andraos-Selim, C, Meidl, J J
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 1989
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC313511/
https://ncbi.nlm.nih.gov/pubmed/2569447
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