Use of the promoter fusion transposon Tn5 lac to identify mutations in Bordetella pertussis vir-regulated genes.

Mutants of Bordetella pertussis deficient in virulence-associated factors were identified by using the transposon Tn5 lac. Tn5 lac is a derivative of Tn5 which generates promoter fusions for beta-galactosidase. Tn5 lac insertions in the vir-regulated genes of B. pertussis were identified by selectin...

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Veröffentlicht in:	Infect Immun
Hauptverfasser:	Weiss, A A, Melton, A R, Walker, K E, Andraos-Selim, C, Meidl, J J
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1989
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC313511/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2569447/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.57.9.2674-2682.1989
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