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Efficient Tn10 transposition into a DNA insertion hot spot in vivo requires the 5-methyl groups of symmetrically disposed thymines within the hot-spot consensus sequence.

Transposon Tn10 inserts preferentially at particular insertion "hot spots" that share a symmetrical 6-base-pair consensus sequence: 5' GCTNAGC 3'. The protein that recognizes this sequence is not known but is likely to be the Tn10-encoded transposase protein. We present evidence...

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Detalhes bibliográficos
Main Authors: Lee, S Y, Butler, D, Kleckner, N
Formato: Artigo
Idioma:Inglês
Publicado em: 1987
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC299434/
https://ncbi.nlm.nih.gov/pubmed/2825167
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