Efficient Tn10 transposition into a DNA insertion hot spot in vivo requires the 5-methyl groups of symmetrically disposed thymines within the hot-spot consensus sequence.
Transposon Tn10 inserts preferentially at particular insertion "hot spots" that share a symmetrical 6-base-pair consensus sequence: 5' GCTNAGC 3'. The protein that recognizes this sequence is not known but is likely to be the Tn10-encoded transposase protein. We present evidence that the 5-methyl gr...
Salvato in:
| Pubblicato in: | Proc Natl Acad Sci U S A |
|---|---|
| Autori principali: | , , |
| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
National Academy of Sciences
1987
|
| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC299434/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2825167/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.22.7876 |
| Tags: |
Nessun Tag, puoi essere il primo ad aggiungerne!!
|