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Quantitation of selective autophagic protein aggregate degradation in vitro and in vivo using luciferase reporters

The analysis of autophagy in cells and tissue has principally been performed via qualitative measures. These assays identify autophagosomes or measure the conversion of LC3I to LC3II. However, qualitative assays fail to quantitate the degradation of an autophagic substrate and therefore only indirec...

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Autores principales: Ju, Jeong-Sun, Miller, Sara E., Jackson, Erin, Cadwell, Ken, Piwnica-Worms, David, Weihl, Conrad C.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2009
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2992796/
https://ncbi.nlm.nih.gov/pubmed/19305149
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