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Segmental isotopic labeling of the Hsp70 molecular chaperone DnaK using expressed protein ligation

Introducing biophysical labels into specific regions of large and dynamic multidomain proteins greatly facilitates mechanistic analysis. Ligation of expressed domains that are labeled in a desired manner before assembly of the intact molecular machine provides such a strategy. We have elaborated an...

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書誌詳細
主要な著者: Clerico, Eugenia M., Zhuravleva, Anastasia, Smock, Robert G., Gierasch, Lila M.
フォーマット: Artigo
言語:Inglês
出版事項: 2010
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC2991546/
https://ncbi.nlm.nih.gov/pubmed/20564022
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bip.21426
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