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Segmental isotopic labeling of the Hsp70 molecular chaperone DnaK using expressed protein ligation
Introducing biophysical labels into specific regions of large and dynamic multidomain proteins greatly facilitates mechanistic analysis. Ligation of expressed domains that are labeled in a desired manner before assembly of the intact molecular machine provides such a strategy. We have elaborated an...
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| Main Authors: | , , , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
2010
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2991546/ https://ncbi.nlm.nih.gov/pubmed/20564022 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/bip.21426 |
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