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An accurate method for determination of receptor-ligand and enzyme-inhibitor dissociation constants from displacement curves.

Receptor-ligand dissociation constants are usually calculated from the displacement curve of a radioactively labeled ligand bound to the receptor. The formula used is restricted to cases in which the concentration of receptor is negligible compared to the concentration of both the displacing ligand...

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Bibliographic Details
Main Authors: Horovitz, A, Levitzki, A
Format: Artigo
Language:Inglês
Published: 1987
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC299141/
https://ncbi.nlm.nih.gov/pubmed/3477796
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