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An accurate method for determination of receptor-ligand and enzyme-inhibitor dissociation constants from displacement curves.
Receptor-ligand dissociation constants are usually calculated from the displacement curve of a radioactively labeled ligand bound to the receptor. The formula used is restricted to cases in which the concentration of receptor is negligible compared to the concentration of both the displacing ligand...
Guardat en:
| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1987
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC299141/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3477796/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.19.6654 |
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