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Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction
The dissociation constant describes the interaction between two partners in the binding equilibrium and is a measure of their affinity. It is a crucial parameter to compare different ligands, e.g., competitive inhibitors, protein isoforms and mutants, for their binding strength to a binding partner....
Enregistré dans:
| Publié dans: | J Vis Exp |
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| Auteur principal: | |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
MyJove Corporation
2018
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC5912426/ https://ncbi.nlm.nih.gov/pubmed/29553569 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57334 |
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