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Titration ELISA as a Method to Determine the Dissociation Constant of Receptor Ligand Interaction

The dissociation constant describes the interaction between two partners in the binding equilibrium and is a measure of their affinity. It is a crucial parameter to compare different ligands, e.g., competitive inhibitors, protein isoforms and mutants, for their binding strength to a binding partner....

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Bibliografische gegevens
Gepubliceerd in:J Vis Exp
Hoofdauteur: Eble, Johannes A.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: MyJove Corporation 2018
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC5912426/
https://ncbi.nlm.nih.gov/pubmed/29553569
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/57334
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