Visualization of the eEF2-80S Ribosome Transition State Complex by Cryo-electron Microscopy

In an attempt to understand ribosome-induced GTP hydrolysis on eEF2, we have determined a 12.6-Å cryo-EM reconstruction of the eEF2-bound 80S ribosome in the presence of aluminum fluoride (AlF(4)(−)) and GDP, with AlF(4)(−) mimicking the γ-phosphate during hydrolysis. This is the first visualization...

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Bibliographic Details
Main Authors: Sengupta, Jayati, Nilsson, Jakob, Gursky, Richard, Kjeldgaard, Morten, Nissen, Poul, Frank, Joachim
Format: Artigo
Language:Inglês
Published: 2008
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2990977/
https://ncbi.nlm.nih.gov/pubmed/18644383
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2008.07.004
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