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Visualization of the eEF2-80S Ribosome Transition State Complex by Cryo-electron Microscopy
In an attempt to understand ribosome-induced GTP hydrolysis on eEF2, we have determined a 12.6-Å cryo-EM reconstruction of the eEF2-bound 80S ribosome in the presence of aluminum fluoride (AlF(4)(−)) and GDP, with AlF(4)(−) mimicking the γ-phosphate during hydrolysis. This is the first visualization...
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| Autors principals: | , , , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2008
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2990977/ https://ncbi.nlm.nih.gov/pubmed/18644383 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2008.07.004 |
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