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Cloning and expression of recombinant, functional ricin B chain.

The cDNA encoding the B chain of the plant toxin ricin has been cloned and expressed in monkey kidney COS-M6 cells. The recombinant B chain was detected by labeling the transfected cells with [35S]methionine and [35S]-cysteine and demonstrating the secretion of a protein with a Mr of 30,000-32,000 t...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Chang, M S, Russell, D W, Uhr, J W, Vitetta, E S
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1987
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC298918/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3112772/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.16.5640
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