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Cloning and expression of recombinant, functional ricin B chain.
The cDNA encoding the B chain of the plant toxin ricin has been cloned and expressed in monkey kidney COS-M6 cells. The recombinant B chain was detected by labeling the transfected cells with [35S]methionine and [35S]-cysteine and demonstrating the secretion of a protein with a Mr of 30,000-32,000 t...
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| Published in: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , , |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
National Academy of Sciences
1987
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC298918/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3112772/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.84.16.5640 |
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