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Mapping the active-site tyrosine of vaccinia virus DNA topoisomerase I.

Site-directed mutagenesis of the vaccinia virus gene encoding a type I DNA topoisomerase implicates Tyr-274 as the active-site residue that forms a covalent adduct with DNA during cycles of DNA-strand breakage and reunion. Replacement of Tyr-274 by phenylalanine results in loss of the ability of the...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Shuman, S, Kane, E M, Morham, S G
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1989
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC298588/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2557629/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.24.9793
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