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RNA structure, not sequence, determines the 5' splice-site specificity of a group I intron.

The group I self-splicing introns act at exon-intron junctions without recognizing a particular sequence. In order to understand splice-site selection, we have developed an assay system based on the Tetrahymena ribozyme to allow the study of numerous 5'-splice-site variants. Cleavage at the cor...

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Detalhes bibliográficos
Publicado no:Proc Natl Acad Sci U S A
Main Authors: Doudna, J A, Cormack, B P, Szostak, J W
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 1989
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC298070/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2678103/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.19.7402
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