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One-sided polymerase chain reaction: the amplification of cDNA.

We report a rapid technique, based on the polymerase chain reaction (PCR), for the direct targeting, enhancement, and sequencing of previously uncharacterized cDNAs. This method is not limited to previously sequenced transcripts, since it requires only two adjacent or partially overlapping specific...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Ohara, O, Dorit, R L, Gilbert, W
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: 1989
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC297692/
https://ncbi.nlm.nih.gov/pubmed/2788276
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