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One-sided polymerase chain reaction: the amplification of cDNA.
We report a rapid technique, based on the polymerase chain reaction (PCR), for the direct targeting, enhancement, and sequencing of previously uncharacterized cDNAs. This method is not limited to previously sequenced transcripts, since it requires only two adjacent or partially overlapping specific...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1989
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC297692/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2788276/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.15.5673 |
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