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One-sided polymerase chain reaction: the amplification of cDNA.

We report a rapid technique, based on the polymerase chain reaction (PCR), for the direct targeting, enhancement, and sequencing of previously uncharacterized cDNAs. This method is not limited to previously sequenced transcripts, since it requires only two adjacent or partially overlapping specific...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Ohara, O, Dorit, R L, Gilbert, W
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1989
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC297692/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2788276/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.15.5673
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