Cargando...

One-sided polymerase chain reaction: the amplification of cDNA.

We report a rapid technique, based on the polymerase chain reaction (PCR), for the direct targeting, enhancement, and sequencing of previously uncharacterized cDNAs. This method is not limited to previously sequenced transcripts, since it requires only two adjacent or partially overlapping specific...

Descrición completa

Gardado en:
Detalles Bibliográficos
Publicado en:Proc Natl Acad Sci U S A
Main Authors: Ohara, O, Dorit, R L, Gilbert, W
Formato: Artigo
Idioma:Inglês
Publicado: National Academy of Sciences 1989
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC297692/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2788276/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.15.5673
Tags: Engadir etiqueta
Sen Etiquetas, Sexa o primeiro en etiquetar este rexistro!