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One-sided polymerase chain reaction: the amplification of cDNA.

We report a rapid technique, based on the polymerase chain reaction (PCR), for the direct targeting, enhancement, and sequencing of previously uncharacterized cDNAs. This method is not limited to previously sequenced transcripts, since it requires only two adjacent or partially overlapping specific...

Täydet tiedot

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Bibliografiset tiedot
Julkaisussa:Proc Natl Acad Sci U S A
Päätekijät: Ohara, O, Dorit, R L, Gilbert, W
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: National Academy of Sciences 1989
Aiheet:
Linkit:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC297692/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2788276/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.86.15.5673
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