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Visualization and enrichment of live putative cancer stem cell populations following p53 inactivation or Bax deletion using non-toxic fluorescent dyes

Putative cancer stem cell (CSC) populations efflux dyes such as Hoechst 33342 giving rise to side populations (SP) that can be analyzed or isolated by flow cytometry. However, Hoechst 33342 is highly toxic, more so to non-SP cells, and thus presents difficulties in interpreting in vivo studies where...

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Bibliographic Details
Main Authors: Allen, Joshua E., Hart, Lori S., Dicker, David T., Wang, Wenge, El-Deiry, Wafik S.
Format: Artigo
Language:Inglês
Published: 2009
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2975270/
https://ncbi.nlm.nih.gov/pubmed/19923899
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