Characterization of PicoGreen Interaction with dsDNA and the Origin of Its Fluorescence Enhancement upon Binding

PicoGreen is a fluorescent probe that binds dsDNA and forms a highly luminescent complex when compared to the free dye in solution. This unique probe is widely used in DNA quantitation assays but has limited application in biophysical analysis of DNA and DNA-protein systems due to limited knowledge...

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Bibliographic Details
Main Authors: Dragan, A.I., Casas-Finet, J.R., Bishop, E.S., Strouse, R.J., Schenerman, M.A., Geddes, C.D.
Format: Artigo
Language:Inglês
Published: The Biophysical Society 2010
Subjects:
Nucleic Acid
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2965993/
https://ncbi.nlm.nih.gov/pubmed/21044599
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.09.012
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