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Characterization of PicoGreen Interaction with dsDNA and the Origin of Its Fluorescence Enhancement upon Binding

PicoGreen is a fluorescent probe that binds dsDNA and forms a highly luminescent complex when compared to the free dye in solution. This unique probe is widely used in DNA quantitation assays but has limited application in biophysical analysis of DNA and DNA-protein systems due to limited knowledge...

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Bibliografische gegevens
Hoofdauteurs: Dragan, A.I., Casas-Finet, J.R., Bishop, E.S., Strouse, R.J., Schenerman, M.A., Geddes, C.D.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: The Biophysical Society 2010
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2965993/
https://ncbi.nlm.nih.gov/pubmed/21044599
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.09.012
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