Characterization of PicoGreen Interaction with dsDNA and the Origin of Its Fluorescence Enhancement upon Binding

PicoGreen is a fluorescent probe that binds dsDNA and forms a highly luminescent complex when compared to the free dye in solution. This unique probe is widely used in DNA quantitation assays but has limited application in biophysical analysis of DNA and DNA-protein systems due to limited knowledge...

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Detalhes bibliográficos
Main Authors: Dragan, A.I., Casas-Finet, J.R., Bishop, E.S., Strouse, R.J., Schenerman, M.A., Geddes, C.D.
Formato: Artigo
Idioma:Inglês
Publicado em: The Biophysical Society 2010
Assuntos:
Nucleic Acid
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2965993/
https://ncbi.nlm.nih.gov/pubmed/21044599
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2010.09.012
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