PicoGreen Assay of Circular DNA for Radiation Biodosimetry

We developed a simple, rapid and quantitative assay using the fluorescent probe PicoGreen to measure the concentration of ionizing radiation-induced double-stranded DNA (dsDNA) in mouse plasma, and we correlated this concentration with the radiation dose. With 70 μl of blood obtained by fingerstick,...

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Bibliografske podrobnosti
izdano v:Radiat Res
Main Authors: Zhang, Steven B., Yang, Shanmin, Vidyasagar, Sadasivan, Zhang, Mei, Casey-Sawicki, Katherine, Liu, Chaomei, Yin, Liangjie, Zhang, Lei, Cao, Yongbing, Tian, Yeping, Swarts, Steven, Fenton, Bruce M., Keng, Peter, Zhang, Lurong, Okunieff, Paul
Format: Artigo
Jezik:Inglês
Izdano: 2015
Teme:
Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4452960/
https://ncbi.nlm.nih.gov/pubmed/25574588
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1667/RR13556.1
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