The –SH Protection Method for Determining Accurate K(d) Values for Enzyme-Coenzyme Complexes of NAD(+)-Dependent Glutamate Dehydrogenase and Engineered Mutants: Evidence for Nonproductive NADPH Complexes

Inactivation rates have been measured for clostridial glutamate dehydrogenase and several engineered mutants at various DTNB concentrations. Analysis of rate constants allowed determination of K(d) for each non-covalent enzyme-DTNB complex and the rate constant for reaction to form the inactive enzy...

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Main Authors: Griffin, Joanna, Engel, Paul C.
Formato: Artigo
Idioma:Inglês
Publicado: SAGE-Hindawi Access to Research 2010
Assuntos:
Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2962915/
https://ncbi.nlm.nih.gov/pubmed/21048875
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4061/2010/951472
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