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An Examination by Site-Directed Mutagenesis of Putative Key Residues in the Determination of Coenzyme Specificity in Clostridial NAD(+)-Dependent Glutamate Dehydrogenase
Sequence and structure comparisons of various glutamate dehydrogenases (GDH) and other nicotinamide nucleotide-dependent dehydrogenases have potentially implicated certain residues in coenzyme binding and discrimination. We have mutated key residues in Clostridium symbiosum NAD(+)-specific GDH to in...
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Main Authors: | , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
SAGE-Hindawi Access to Research
2011
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3157743/ https://ncbi.nlm.nih.gov/pubmed/21876794 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4061/2011/595793 |
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