Fluorescence-based directed termination PCR: direct mutation characterization without sequencing

We describe a fluorescence-based directed termination PCR (fluorescent DT–PCR) that allows accurate determination of actual sequence changes without dideoxy DNA sequencing. This is achieved using near infrared dye-labeled primers and performing two PCR reactions under low and unbalanced dNTP concent...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Chen, Junjian Z., Smith, Leslie, Pfeifer, Gerd P., Holmquist, Gerald P.
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Oxford University Press 2001
Gaiak:
NAR Methods Online
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC29624/
https://ncbi.nlm.nih.gov/pubmed/11160937
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