Fluorescence-based directed termination PCR: direct mutation characterization without sequencing

We describe a fluorescence-based directed termination PCR (fluorescent DT–PCR) that allows accurate determination of actual sequence changes without dideoxy DNA sequencing. This is achieved using near infrared dye-labeled primers and performing two PCR reactions under low and unbalanced dNTP concent...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Chen, Junjian Z., Smith, Leslie, Pfeifer, Gerd P., Holmquist, Gerald P.
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 2001
Assuntos:	NAR Methods Online
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC29624/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11160937/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/29.4.e17
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