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Directed termination PCR: a one-step approach to mutation detection.

We describe a novel PCR-based method that allows the generation of nested termination fragments by integrating both selective DNA amplification and directed chain termination into a single PCR reaction. These termination fragments can be examined for sequence variation in either denaturing or non-de...

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Detalhes bibliográficos
Main Authors: Chen, J, Hebert, P D
Formato: Artigo
Idioma:Inglês
Publicado em: 1998
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC147424/
https://ncbi.nlm.nih.gov/pubmed/9490805
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