Mechanism and Stereospecificity of a Fully Saturating Polyketide Synthase Module: Nanchangmycin Synthase Module 2 and Its Dehydratase Domain

[Image: see text] Recombinant nanchangmycin synthase module 2 (NANS module 2), with the thioesterase domain from the 6-deoxyerythronolide B synthase (DEBS TE) appended to the C-terminus, was cloned and expressed in Escherichia coli. Incubation of NANS module 2+TE with (±)-2-methyl-3-keto-butyryl-N-a...

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Autores principales: Guo, Xun, Liu, Tiangang, Valenzano, Chiara R., Deng, Zixin, Cane, David E.
Formato: Artigo
Lenguaje:Inglês
Publicado: 2010
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Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2959152/
https://ncbi.nlm.nih.gov/pubmed/20925339
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja1073432
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