A feeder-free culture using autogeneic conditioned medium for undifferentiated growth of human embryonic stem cells: Comparative expression profiles of mRNAs, microRNAs and proteins among different feeders and conditioned media

BACKGROUND: Human embryonic stem (hES) cell lines were derived from the inner cell mass of human blastocysts, and were cultured on mouse embryonic fibroblast (MEF) feeder to maintain undifferentiated growth, extensive renewal capacity, and pluripotency. The hES-T3 cell line with normal female karyot...

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Detalhes bibliográficos
Main Authors: Tsai, Zong-Yun, Singh, Sher, Yu, Sung-Liang, Chou, Chi-Hsien, Li, Steven Shoei-Lung
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2010
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Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2958897/
https://ncbi.nlm.nih.gov/pubmed/20937144
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2121-11-76
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