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Feeder & basic fibroblast growth factor-free culture of human embryonic stem cells: Role of conditioned medium from immortalized human feeders

BACKGROUND & OBJECTIVES: Human embryonic stem cell (hESC) lines are commonly maintained on inactivated feeder cells, in the medium supplemented with basic fibroblast growth factor (bFGF). However, limited availability of feeder cells in culture, and the high cost of growth factors limit their us...

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Vydáno v:Indian J Med Res
Hlavní autoři: Teotia, Pooja, Sharma, Shilpa, Airan, Balram, Mohanty, Sujata
Médium: Artigo
Jazyk:Inglês
Vydáno: Medknow Publications & Media Pvt Ltd 2016
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC5433277/
https://ncbi.nlm.nih.gov/pubmed/28474621
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4103/ijmr.IJMR_424_15
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