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Feeder & basic fibroblast growth factor-free culture of human embryonic stem cells: Role of conditioned medium from immortalized human feeders

BACKGROUND & OBJECTIVES: Human embryonic stem cell (hESC) lines are commonly maintained on inactivated feeder cells, in the medium supplemented with basic fibroblast growth factor (bFGF). However, limited availability of feeder cells in culture, and the high cost of growth factors limit their us...

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Detalles Bibliográficos
Publicado en:Indian J Med Res
Main Authors: Teotia, Pooja, Sharma, Shilpa, Airan, Balram, Mohanty, Sujata
Formato: Artigo
Idioma:Inglês
Publicado: Medknow Publications & Media Pvt Ltd 2016
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC5433277/
https://ncbi.nlm.nih.gov/pubmed/28474621
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4103/ijmr.IJMR_424_15
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