High-throughput knockout screen in fission yeast

We have designed the most efficient strategy to knock out genes in fission yeast Schizosaccharomyces pombe on a large scale. Our technique is based on knockout constructs that contain regions homologous to the target gene cloned into vectors carrying dominant drug-resistance markers. Most of the ste...

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Hlavní autoři: Gregan, Juraj, Rabitsch, Peter K, Rumpf, Cornelia, Novatchkova, Maria, Schleiffer, Alexander, Nasmyth, Kim
Médium: Artigo
Jazyk:Inglês
Vydáno: 2006
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Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2957175/
https://ncbi.nlm.nih.gov/pubmed/17406492
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2006.385
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