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High-throughput knockout screen in fission yeast
We have designed the most efficient strategy to knock out genes in fission yeast Schizosaccharomyces pombe on a large scale. Our technique is based on knockout constructs that contain regions homologous to the target gene cloned into vectors carrying dominant drug-resistance markers. Most of the ste...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2957175/ https://ncbi.nlm.nih.gov/pubmed/17406492 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2006.385 |
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