Isolation of Specific Clones from Nonarrayed BAC Libraries through Homologous Recombination

We have developed a new approach to screen bacterial artificial chromosome (BAC) libraries by recombination selection. To test this method, we constructed an orangutan BAC library using an E. coli strain (DY380) with temperature inducible homologous recombination (HR) capability. We amplified one li...

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Bibliografiske detaljer
Main Authors: Nefedov, Mikhail, Carbone, Lucia, Field, Matthew, Schein, Jacquie, de Jong, Pieter J.
Format: Artigo
Sprog:Inglês
Udgivet: Hindawi Publishing Corporation 2011
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2957146/
https://ncbi.nlm.nih.gov/pubmed/20981149
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2011/560124
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