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Engineering EGFP reporter constructs into a 200 kb human β-globin BAC clone using GET Recombination

GET Recombination, a simple inducible homologous recombination system for Escherichia coli, was used to target insertion of an EGFP cassette between the start and termination codons of the β-globin gene in a 200 kb BAC clone. The high degree of homology between the promoter regions of the β- and δ-g...

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Detalhes bibliográficos
Main Authors: Orford, Michael, Nefedov, Mikhail, Vadolas, Jim, Zaibak, Faten, Williamson, Robert, Ioannou, Panayiotis A.
Formato: Artigo
Idioma:Inglês
Publicado em: Oxford University Press 2000
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC110756/
https://ncbi.nlm.nih.gov/pubmed/10982895
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