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Insertional mutagenesis based on illegitimate recombination in Schizosaccharomyces pombe

An efficient insertional mutagenesis system has been developed for Schizosaccharomyces pombe based on linear PCR-generated cassettes containing selectable markers. It depends upon illegitimate recombination for integration into the genome. Various selectable markers of different sizes can be used to...

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Detalles Bibliográficos
Main Authors: Chua, G., Taricani, L., Stangle, W., Young, P. G.
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 2000
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC102638/
https://ncbi.nlm.nih.gov/pubmed/10871352
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